![]() ![]() The number of dilutions is equal to the number of times the dilution factor will be multiplied by itself to equal the starting concentration divided by the final concentration. For example, a DF of 100 means a 1:100 dilution. The dilution factor is often used as the denominator of a fraction. To bring this 2 ml sample up to a total volume of 10 ml, you must add 10 ml – 2 ml = 8 ml diluent. If you need 10 ml, final volume, then you need 1/5 of 10 ml = 2 ml sample. What is the dilution factor for 1 5?Īnswer: 1:5 dilution = 1/5 dilution = 1 part sample and 4 parts diluent in a total of 5 parts. When performing a dilution there is a equation that can be used to determine the final concentration. ![]() Why do we use dilution factor?īy performing a dilution on a sample it may reduce the interfering substance to a point where it no longer interferes with the test. For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DF=ViVf = 1mL10mL=110. The dilution factor or the dilution is the initial volume divided by the final volume. In serial dilutions, you multiply the dilution factors for each step. How do you calculate the dilution factor of a serial dilution? ![]()
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